FastQCFastQC Report
Thu 2 Jun 2016
DRR025054_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025054_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3411974
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115550.33866025942753375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100350.2941112681397924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68830.20173072831152877No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62100.055.5818141
GTATCAA116650.041.3206751
ATCAACG150750.031.6791573
TCAACGC152750.031.3534744
TATCAAC156400.030.5948492
GTGGTAT28050.030.5117281
CAACGCA158900.030.3174485
AACGCAG166750.029.0593286
TGGTATC29250.027.4794162
ACAACGC6900.026.5676293
TACCTGG29550.025.9279022
TAACGCA3700.025.4051044
ACGCAGA189950.025.2124397
TATAACG3000.025.0689412
CGCAGAG192550.024.7248238
GCAGAGT204300.022.8887449
GTACCTG34200.022.549971
GTACATG102200.022.4081691
AGAGTAC194200.020.64272710-11
TACATGG102350.020.4825612
Produced by FastQC (version 0.11.5)