FastQCFastQC Report
Thu 2 Jun 2016
DRR025053_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025053_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221209
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91740.2847998996650016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74530.23137275476381694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47200.14652883436001826No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52350.057.476391
GTATCAA102700.043.7636641
ATCAACG127350.034.7319533
TCAACGC129500.034.1184964
TATCAAC131650.033.7455832
CAACGCA135600.032.5490045
AACGCAG140800.031.4431536
GTGGTAT21000.029.999341
ACGCAGA156800.027.8741257
TGGTATC22250.027.6787992
CGCAGAG157500.027.6602978
ACAACGC6200.026.5347423
GTCTCGC8900.025.8840521
GCAGAGT169600.025.2435369
TACCGTC1351.4593741E-524.368497
GTACATG85150.023.4655341
TCTGTCG8900.022.7057618
TACATGG84700.022.2015132
AGAGTAC160400.021.41759110-11
CAGAGTA166550.021.26161410-11
Produced by FastQC (version 0.11.5)