FastQCFastQC Report
Thu 2 Jun 2016
DRR025053_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025053_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221209
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100970.3134537374010814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87520.27169922845738975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61290.19027017495604912No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58650.059.1715471
GTATCAA109600.040.9319421
ATCAACG141650.031.5597363
TGGTATC23500.031.4056642
GTGGTAT25050.031.161971
TCAACGC143300.031.1239764
TATCAAC146550.030.4729122
CAACGCA149000.030.1541215
ACAACGC5650.029.9518383
AACGCAG155750.028.9675316
TATAACG3650.025.758072
ACGCAGA176250.025.3841517
CGCAGAG177100.025.2357838
GCAGAGT186850.023.2147189
GTACATG81550.023.1230431
TACATGG79900.021.5921292
GTACCGG2859.640644E-1121.4497911
AGAGTAC178950.021.32451210-11
TACCTGG21900.021.2504062
GTACCTG23350.020.7431581
Produced by FastQC (version 0.11.5)