FastQCFastQC Report
Thu 2 Jun 2016
DRR025052_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025052_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5780715
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142180.24595573384953245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115550.19988876808491682No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73140.12652414104483617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98900.053.0073051
GTATCAA188650.042.094951
ATCAACG234050.033.624453
TCAACGC236800.033.3122184
TATCAAC248600.031.8102462
CAACGCA248500.031.7237825
AACGCAG260250.030.4317786
ACGCAGA289500.027.0474177
CGCAGAG289950.026.777818
GTGGTAT46150.026.2848321
GTACATG217850.026.0931151
TACATGG210950.025.0066472
TGGTATC47250.024.875992
GCAGAGT312050.024.309029
ACATGGG218600.023.2264023
AGAGTAC290150.021.02520410-11
GAGTACT188450.020.83625412-13
CAGAGTA307300.020.35652410-11
AGTACTT195200.019.5860612-13
CATGGGG123500.019.3751094
Produced by FastQC (version 0.11.5)