FastQCFastQC Report
Thu 2 Jun 2016
DRR025052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5780715
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155360.26875568160686003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129620.22422831777729918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89140.15420237807952822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101750.056.536271
GTATCAA193800.041.9882131
ATCAACG249550.032.325893
TCAACGC253450.031.8207634
TATCAAC263750.030.7105352
CAACGCA266300.030.3382325
AACGCAG278200.029.1078416
GTGGTAT44150.026.3148781
ACGCAGA316150.025.479347
CGCAGAG315050.025.26938
GTACATG215450.025.0846821
TACATGG209050.024.2192152
TGGTATC46350.024.0377142
GCAGAGT337150.022.9577739
ACATGGG213000.022.7104933
AGAGTAC316850.020.60934410-11
GAGTACT211500.020.42040812-13
AGTACTT217350.019.91403612-13
CAGAGTA333900.019.57103710-11
CATGGGG122750.019.373794
Produced by FastQC (version 0.11.5)