FastQCFastQC Report
Thu 2 Jun 2016
DRR025051_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025051_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585889
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189620.5287949515447913No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150530.41978432684335737No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98400.2744089401540315No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43850.12228487831051099No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40130.11191088179249274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84850.058.6233751
GTATCAA169600.044.9914171
TACCTGG29100.037.1596682
ATCAACG216450.034.8602263
GTGGTAT32950.034.6727141
TCAACGC219950.034.219534
TGGTATC31850.033.5083772
TATCAAC226800.033.3748862
TATAACG4600.032.7061272
CAACGCA231150.032.6414645
AACGCAG242950.031.0526166
GTACCTG36700.030.8736481
ACCTGGG34300.030.0166873
GTACATG201850.028.3464581
TACATGG197950.027.693642
CGCAGAG269250.027.6152158
ACGCAGA270500.027.6099997
GTACACG2750.027.3542161
GCAGAGT282500.025.7539659
ACATGGG205700.025.7345683
Produced by FastQC (version 0.11.5)