FastQCFastQC Report
Thu 2 Jun 2016
DRR025051_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025051_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3585889
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205120.5720199370365341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168020.46855884273049164No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119920.33442195226901894No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45800.1277228603562464No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39650.11057230159661942No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39010.10878752800212166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96050.059.4076581
GTATCAA183400.042.5525931
TACCTGG28350.035.652642
ATCAACG240150.032.2025033
TATAACG3800.032.1659242
TCAACGC244250.031.6732434
TATCAAC248950.031.1019612
CAACGCA254000.030.457445
AACGCAG264700.029.3505486
GTACCTG36600.029.3043941
GTGGTAT37100.028.782661
ACCTGGG35150.027.6854323
TGGTATC38650.026.8812272
GTACATG198950.026.7895181
TACATGG196150.025.956522
ACGCAGA300100.025.7302887
CGCAGAG298650.025.6974878
GTACCGG2601.8189894E-1225.329911
TACAACG8500.024.3354872
ACATGGG203650.024.2388063
Produced by FastQC (version 0.11.5)