FastQCFastQC Report
Thu 2 Jun 2016
DRR025050_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025050_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397452
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70460.29389535223228663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59310.24738764321454612No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35270.14711451991531008No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46350.049.6040081
GTATCAA82000.038.76061
ATCAACG97550.032.24123
TCAACGC99000.031.9575964
TATCAAC102350.030.9620512
CAACGCA103250.030.7332125
AACGCAG107100.029.8002686
TACCTGG19500.027.4870382
ACGCAGA117350.026.8357337
GTACCTG21600.026.5560671
CGCAGAG118450.026.5451778
TATAACG2451.2732926E-1124.9479522
TAACGCA2850.024.7423324
GCAGAGT127550.024.171299
GTACCGG2602.7284841E-1123.5086481
TCTGTCG7350.023.0202338
ATAACGC2555.5842975E-1022.123483
GTACATG60600.022.1120971
TGGTATC23650.021.4709012
GAGTACT91950.021.31268312-13
Produced by FastQC (version 0.11.5)