FastQCFastQC Report
Thu 2 Jun 2016
DRR025050_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025050_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397452
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80850.3372330290658582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69300.2890568820564499No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46950.19583290927201047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50600.047.690431
GTATCAA90900.038.4493831
ATCAACG112250.030.5691763
TCAACGC113500.030.185434
TATCAAC116700.029.4846842
CAACGCA117700.029.1881485
AACGCAG121350.028.348956
TACCTGG21600.027.4203552
GTACCTG23000.026.9966871
TAACGCA2104.9112714E-1126.8551544
TATAACG2357.2759576E-1226.0034962
ACGCAGA134900.025.3969337
CGCAGAG135200.024.9919288
ATAACGC3200.024.9715883
GTCTCGC7300.023.8420311
GTACATG61500.023.7875461
ACAACGC4350.023.7728163
GCAGAGT142600.023.1018129
TACATGG62350.022.2403582
AGAGTAC125650.022.01058210-11
Produced by FastQC (version 0.11.5)