FastQCFastQC Report
Thu 2 Jun 2016
DRR025049_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025049_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607605
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87190.24168388723266543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78530.21767904191284798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44370.1229901832379099No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62150.056.5109831
GTATCAA116000.041.2207951
ATCAACG143550.033.0435833
TCAACGC144850.032.746574
TATCAAC149800.031.7931042
CAACGCA151600.031.3505465
AACGCAG157150.030.4186216
CGCAGAG172100.027.36358
ACGCAGA173450.027.2329447
GTGGTAT29800.024.612841
GCAGAGT187750.024.4314179
TGGTATC29050.023.9525032
GTACATG100100.023.9076611
TACATGG96150.022.639512
GAGTACT134200.021.39771812-13
ACATGGG100000.020.9668923
CAGAGTA186650.020.94966510-11
AGAGTAC175450.020.84049410-11
AGTACTT139300.019.65276112-13
GTACTTT147350.019.60002114-15
Produced by FastQC (version 0.11.5)