FastQCFastQC Report
Thu 2 Jun 2016
DRR025049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3607605
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93230.2584262966705058No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85570.237193373443046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51510.1427817069773437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64200.053.487891
GTATCAA117100.039.2870671
ATCAACG144750.031.340463
TCAACGC146500.031.0233824
TATCAAC151050.030.251592
CAACGCA151850.029.8684585
AACGCAG159550.028.4855046
GTGGTAT30100.027.5051361
TGGTATC29800.026.5030382
CGCAGAG179500.024.9774518
ACGCAGA180900.024.9670417
TATAACG3300.022.7933772
ATAACGC2905.456968E-1222.6948153
GCAGAGT194650.022.64719
GTACATG97950.021.6590651
GTCTCGC10050.021.5307641
TACATGG95900.021.1281432
GAGTACT135300.020.75411812-13
AGAGTAC178850.020.61427910-11
TCTGTCG9200.020.4331998
Produced by FastQC (version 0.11.5)