FastQCFastQC Report
Thu 2 Jun 2016
DRR025048_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025048_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600461
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206080.5723711491389575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157520.43749953131001834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108150.300378201569188No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49690.13801010481713313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41510.11529079192914463No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40740.11315217690179118No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97150.060.152381
GTATCAA194200.043.4307861
TACCTGG31700.040.3392752
ACCTGGG35900.035.2241133
ATCAACG246050.034.0842483
TCAACGC250400.033.565364
TATCAAC258900.032.5586742
CAACGCA261600.032.325075
GTACCTG41550.031.795071
GTACCGG3450.031.3424721
AACGCAG273000.031.1280336
GTGGTAT36050.031.0381741
TGGTATC36300.030.5649592
TACAACG7850.030.5435122
GTACATG213600.028.1730081
ACGCAGA299100.028.0177157
CGCAGAG297350.027.9925448
TACATGG208250.027.6547362
GCAGAGT309050.026.3389729
ACATGGG221300.025.1933273
Produced by FastQC (version 0.11.5)