FastQCFastQC Report
Thu 2 Jun 2016
DRR025048_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025048_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3600461
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231700.6435287036854447No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183220.5088792796255813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137670.38236770235811474No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47280.13131651752372822No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45900.1274836750071727No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45300.1258172217391051No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38020.105597588753218No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103000.058.305541
TATAACG4700.044.0059972
GTATCAA199250.042.2012521
TACAACG7750.038.818192
TACCTGG30400.037.7288252
ACAACGC9850.033.4055673
ACCTGGG34700.031.9697743
ATCAACG264100.031.6283133
TCAACGC268950.031.052354
GTACCTG38200.031.023681
TAACGCA6850.030.8745444
GTGGTAT38200.030.777461
TGGTATC38450.030.6855832
TATCAAC278400.030.1219182
CAACGCA279400.029.991875
AACGCAG294250.028.6220116
GTACATG217500.027.0275651
TACATGG212950.026.5107732
ACGCAGA327150.025.5708797
CGCAGAG327000.025.452558
Produced by FastQC (version 0.11.5)