FastQCFastQC Report
Thu 2 Jun 2016
DRR025047_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025047_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4806941
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110000.2288357606219839No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93300.19409433150937364No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55500.11545804285927372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75800.052.972821
GTATCAA140000.041.2751161
ATCAACG168550.033.97263
TCAACGC170250.033.602964
CAACGCA176600.032.4745525
TATCAAC181100.031.7514382
AACGCAG184350.031.156126
ACGCAGA208050.027.1763177
CGCAGAG209750.026.8429058
GTGGTAT34700.026.2867371
GTACATG133850.025.5376221
GCAGAGT223700.024.3706269
TGGTATC37150.024.0464062
TACATGG135400.023.5779762
ACATGGG137750.022.285983
GAGTACT154050.021.03391612-13
CAGAGTA221100.020.58664310-11
TACCTGG37050.020.5580642
AGAGTAC211200.020.45013810-11
GTACTTT169450.018.9014214-15
Produced by FastQC (version 0.11.5)