FastQCFastQC Report
Thu 2 Jun 2016
DRR025047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4806941
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121580.25292592524018914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106170.22086811550214575No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70640.14695416482124493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74150.054.7451131
GTATCAA139100.041.6609651
ATCAACG176300.032.63823
TCAACGC176900.032.5997624
TATCAAC182300.031.7187142
CAACGCA184950.031.1046125
AACGCAG192750.030.0897456
ACGCAGA222100.025.7732587
CGCAGAG224600.025.38078
ACAACGC8250.025.0723953
GTACATG136950.024.523471
GTGGTAT35100.023.5858361
TACATGG136600.023.4365432
TGGTATC34300.023.1627272
GCAGAGT242400.023.032259
ACATGGG139150.022.1624493
AGAGTAC225850.020.6832510-11
TACCTGG35550.020.4969482
GAGTACT169300.020.12489312-13
CAGAGTA238550.019.58211110-11
Produced by FastQC (version 0.11.5)