FastQCFastQC Report
Thu 2 Jun 2016
DRR025046_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025046_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714258
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98670.26565198217248237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85590.23043633479419037No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49400.13300099239202015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA60300.055.750061
GTATCAA115650.042.64681
ATCAACG141500.034.122083
TCAACGC143300.033.758184
TATCAAC147200.032.9618032
CAACGCA148500.032.670615
GTGGTAT27300.032.0336881
AACGCAG154250.031.56876
TGGTATC27800.029.2577132
ACGCAGA174150.027.689987
CGCAGAG173900.027.512838
GTACATG92150.025.4602011
GCAGAGT186900.024.6938749
TACCTGG26850.023.8140742
TACATGG92850.023.79882
ACAACGC6000.022.7231373
ACATGGG94050.022.4444563
AGAGTAC176500.021.24932910-11
GTACCTG31950.021.0436861
GTCTCGC9050.020.7810761
Produced by FastQC (version 0.11.5)