FastQCFastQC Report
Thu 2 Jun 2016
DRR025046_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025046_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3714258
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109080.29367911437493033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99280.2672943021190235No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62170.1673820181581355No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63100.057.397281
GTATCAA114750.040.539051
ATCAACG145050.031.5992643
TCAACGC146000.031.3873144
TATCAAC152750.030.1294752
CAACGCA153550.029.8746195
AACGCAG160950.028.5587086
TGGTATC26550.028.1528722
GTGGTAT27750.028.136821
TATAACG4200.026.862852
ACGCAGA183200.024.8839557
CGCAGAG182800.024.8348988
ATAACGC3900.024.1076833
GTACATG97650.024.0839481
TACATGG98150.022.8464222
GCAGAGT197000.022.7346429
ACATGGG101150.021.1928373
ACAACGC7100.020.5254863
AGAGTAC186750.020.47191210-11
TACCTGG27650.020.0621282
Produced by FastQC (version 0.11.5)