FastQCFastQC Report
Thu 2 Jun 2016
DRR025045_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025045_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408171
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91850.26949938838162757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79880.23437791120222545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46430.13623142735502414No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.053.1813931
GTATCAA103950.040.521031
ATCAACG128850.032.2871973
GTGGTAT25250.031.836971
TCAACGC130800.031.6971024
TATCAAC132200.031.6482092
CAACGCA136600.030.5228635
TACCTGG26200.029.9644152
TATAACG4000.029.3812732
AACGCAG143700.029.1757626
ATAACGC3250.028.927983
GTACCTG29050.027.672411
ACAACGC6650.026.8616963
ACGCAGA159350.025.867177
TCTGTCG10400.025.759078
TGGTATC29100.025.6858962
CGCAGAG159800.025.6171048
GTCTCGC10300.025.559231
GTACATG80250.024.3694231
GCAGAGT170950.023.368919
Produced by FastQC (version 0.11.5)