FastQCFastQC Report
Thu 2 Jun 2016
DRR025045_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025045_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3408171
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103880.3047969130656883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90390.2652155657682669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60440.17733851969282058No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57700.057.1443941
GTATCAA108200.041.167471
TATAACG3850.032.9708752
ATCAACG134800.032.6447533
ATAACGC3900.032.5481723
TCAACGC136800.032.225824
TATCAAC140850.031.3760622
CAACGCA142750.030.9813775
AACGCAG150350.029.5711736
GTGGTAT25400.028.703141
TGGTATC24800.027.4880372
CGCAGAG168700.025.9352198
TACCTGG28100.025.9330022
ACGCAGA170300.025.9134627
GTCTCGC10000.024.9291421
TAACGCA5700.024.7362374
GTACCTG30800.024.4343471
GCAGAGT180900.023.6665619
GTATAAC9600.023.5180551
GTACATG79550.022.2911571
Produced by FastQC (version 0.11.5)