FastQCFastQC Report
Thu 2 Jun 2016
DRR025044_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025044_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4498235
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108500.24120571735358423No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89990.20005624428247967No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58210.12940631158665566No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95050.042.594571
GTATCAA162350.036.204361
ATCAACG195650.029.7259043
TCAACGC199050.029.2631174
TATCAAC205050.028.5256022
CAACGCA205750.028.2870335
AACGCAG213900.027.3127576
ACGCAGA236000.024.43487
CGCAGAG236150.024.3192318
GTACATG136800.024.0267091
TACATGG133750.023.1667882
GCAGAGT250050.021.9900199
ACATGGG136500.021.871893
GAGTACT149750.021.19900512-13
AGAGTAC213900.020.9386510-11
GTACCTG34650.020.6273561
TACCTGG33900.020.250112
GTACCGG3303.274181E-1119.9488241
AGTACTT161650.019.3767712-13
GTACTTT167400.019.06233414-15
Produced by FastQC (version 0.11.5)