FastQCFastQC Report
Thu 2 Jun 2016
DRR025044_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025044_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4498235
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124700.2772198428939351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103230.22949001108212444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71700.15939585192858977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97800.045.058411
GTATCAA169750.037.2915461
ATCAACG210500.029.6127133
TCAACGC212500.029.328464
TATCAAC218900.028.5193182
CAACGCA221050.028.3003755
AACGCAG229000.027.5837926
ACGCAGA254350.024.5753927
CGCAGAG253900.024.4515368
GTACATG134500.023.7422891
GCAGAGT269000.022.4150879
TACATGG130300.022.2961982
AGAGTAC233950.021.38398710-11
ACATGGG135450.020.8584613
GAGTACT172450.020.6163512-13
AGTACTT180450.020.10603712-13
TACCTGG35500.019.7307992
GTACCTG36200.019.6174931
TCTGTCG10700.019.3256878
CAGAGTA267450.018.8021410-11
Produced by FastQC (version 0.11.5)