FastQCFastQC Report
Thu 2 Jun 2016
DRR025043_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025043_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4598969
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119040.2588406227569701No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98850.21493947882666745No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58610.1274416070210519No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74100.053.299911
GTATCAA135100.040.8580931
ATCAACG167950.032.4441033
TCAACGC168950.032.2224854
CAACGCA176900.030.8796835
TATCAAC178750.030.591382
AACGCAG185600.029.451446
GTGGTAT33300.028.80391
GTACATG149500.027.6447751
TGGTATC34700.027.0997872
TACATGG145750.026.388222
ACGCAGA209650.025.7814757
CGCAGAG210050.025.5970128
ACATGGG149450.024.4117053
GCAGAGT226250.023.2448869
AGAGTAC210550.020.39103310-11
GAGTACT152600.020.2188612-13
TACCTGG35400.020.1885762
CAGAGTA222100.019.90197210-11
CATGGGG85350.019.8293114
Produced by FastQC (version 0.11.5)