FastQCFastQC Report
Thu 2 Jun 2016
DRR025043_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025043_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4598969
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133100.2894126922795087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113170.24607689245132988No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75330.16379758158839514No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78750.057.094131
GTATCAA143650.041.0559431
ATCAACG183200.031.585663
TCAACGC185300.031.1718834
TATCAAC192750.030.1917532
CAACGCA193450.029.8586165
AACGCAG201900.028.7017656
GTGGTAT35250.027.885041
TGGTATC34500.027.6591642
GTACATG147900.026.2979241
TACATGG143600.025.2711472
ACGCAGA228200.025.1052867
CGCAGAG228300.024.8469898
TATAACG3250.024.5882532
ACATGGG148800.023.8191243
GCAGAGT245500.022.4938329
GAGTACT170250.020.63484612-13
CATGGGG86350.020.5738724
TACCTGG36250.020.4885482
AGAGTAC230450.020.29193710-11
Produced by FastQC (version 0.11.5)