FastQCFastQC Report
Thu 2 Jun 2016
DRR025042_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025042_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328577
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83690.2514287637029277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70440.21162196337954625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42720.12834313281621545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49950.050.2587171
GTATCAA90400.041.1872061
TCAACGC111300.032.9849474
ATCAACG112800.032.587993
CAACGCA117850.031.2300385
TATCAAC118650.031.1029072
AACGCAG122400.030.1422986
TACCTGG27700.028.8514882
GTGGTAT23450.028.2671411
GTACATG109200.025.9166851
ACGCAGA141650.025.7142147
TACATGG108750.025.0292822
CGCAGAG147150.024.5607248
TGGTATC26850.024.5122322
ACATGGG109250.023.8798453
GTACCTG36250.023.3437211
GCAGAGT159950.021.5374779
ACCTGGG35400.021.5115493
TAACGCA3550.021.1861254
TATAACG3800.021.0312162
Produced by FastQC (version 0.11.5)