FastQCFastQC Report
Thu 2 Jun 2016
DRR025042_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025042_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3328577
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95230.2860982335694803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81450.2446991612331636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55770.1675490757762251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52850.049.0429761
GTATCAA96600.041.878541
TCAACGC122400.032.5242044
ATCAACG122850.032.3726433
TATCAAC127200.031.3769022
CAACGCA127350.031.1861975
TACCTGG29350.030.5924852
AACGCAG131950.030.2766386
GTGGTAT24600.029.065561
ACAACGC6450.028.4241753
TACAACG7150.026.2992532
ACGCAGA152700.025.945027
TGGTATC26550.025.8510272
CGCAGAG158650.024.645398
ACCTGGG36300.024.6053713
GTACATG105950.024.1528361
GTACCTG38650.023.8548551
TACATGG106850.022.7020282
GCAGAGT172050.022.2615499
ACATGGG107400.022.0164183
Produced by FastQC (version 0.11.5)