FastQCFastQC Report
Thu 2 Jun 2016
DRR025041_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025041_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5328634
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109470.20543726591092576No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91650.17199529935814695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53990.1013205260485145No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78450.049.2652051
GTATCAA147000.041.2604331
ATCAACG178400.033.5200583
TCAACGC180450.033.1640624
TATCAAC187450.032.1552432
CAACGCA188300.031.9556565
AACGCAG196700.030.5875536
ACGCAGA221700.027.051517
CGCAGAG222450.026.8108928
GTACATG139500.024.4694291
GCAGAGT243050.023.7648059
GTGGTAT43800.023.1868131
TACATGG140800.022.907132
TGGTATC41600.022.151952
ACATGGG143700.021.4941923
GAGTACT158750.020.66405912-13
AGAGTAC220600.020.54853210-11
TACCTGG41800.019.6838912
CAGAGTA238550.019.38651810-11
GTACCTG44600.019.2920191
Produced by FastQC (version 0.11.5)