FastQCFastQC Report
Thu 2 Jun 2016
DRR025041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5328634
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122380.22966486345280984No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107410.201571359564196No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67640.1269368472295151No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA87000.050.934051
GTATCAA158950.039.923441
ATCAACG196550.031.7652913
TCAACGC198900.031.3338874
TATCAAC208450.030.0877652
CAACGCA209000.029.9096185
AACGCAG216800.028.898036
ACGCAGA245500.025.2509927
GTGGTAT44850.025.0675581
CGCAGAG245500.025.0392328
GTACATG144150.023.9855271
TGGTATC44950.022.8014662
GCAGAGT267200.022.5308449
TATAACG4800.022.5279962
TACATGG142900.022.4053042
GTACCTG43400.020.9191551
ACATGGG145950.020.8736673
AGAGTAC244750.020.39231910-11
TACCTGG43400.019.7159462
GAGTACT180650.019.6934912-13
Produced by FastQC (version 0.11.5)