FastQCFastQC Report
Thu 2 Jun 2016
DRR025040_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025040_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3932132
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94650.2407091114947311No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84240.21423492395474006No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47150.1199095045639363No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67900.056.5743941
GTATCAA127450.041.8719941
ATCAACG153600.034.372573
TCAACGC156400.033.726714
TATCAAC162200.032.7840652
CAACGCA162950.032.5717355
AACGCAG170400.031.3356196
GTGGTAT31950.028.9909881
TGGTATC30650.028.071892
ACGCAGA188450.027.9323337
CGCAGAG188050.027.9164148
GCAGAGT203050.025.298639
GTACATG106350.024.4486731
ACAACGC7500.023.8202193
TATAACG4150.023.7915252
TACATGG108700.022.5350862
ACATGGG109050.021.6853143
GAGTACT138950.021.42677512-13
CAGAGTA201750.021.17546310-11
AGAGTAC189200.020.99027810-11
Produced by FastQC (version 0.11.5)