FastQCFastQC Report
Thu 2 Jun 2016
DRR025040_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025040_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3932132
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107960.2745584329315496No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95630.24320139812193486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60790.1545980653752214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66800.055.5560461
GTATCAA125900.041.8056641
ATCAACG160050.032.693833
TCAACGC162000.032.3778844
TATCAAC167850.031.454642
CAACGCA169450.031.176265
AACGCAG176000.030.0686516
GTGGTAT31500.028.6695881
TGGTATC31100.027.361792
ACGCAGA200850.026.1137687
CGCAGAG202850.025.6702948
ATAACGC4200.023.5069523
TATAACG4000.023.506952
GCAGAGT217750.023.3741829
ACAACGC7450.023.3491883
TACAACG8400.022.3875732
AGAGTAC205250.021.11124610-11
TACCTGG32300.020.9597592
TAACGCA4050.020.8889564
GTACATG105900.020.8308831
Produced by FastQC (version 0.11.5)