FastQCFastQC Report
Thu 2 Jun 2016
DRR025039_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025039_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3484934
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99080.28430954503012107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80490.23096563665194236No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49450.14189651798283698No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75250.040.4873961
GTATCAA123550.035.9997751
ACAACGC8250.035.3296433
TACCTGG36100.034.6433262
TATAACG4750.034.6433262
GTACCTG38550.033.0518881
TACAACG9200.031.1736132
ATCAACG147950.029.455493
TCAACGC148500.029.281824
ATAACGC4900.028.7823753
TATCAAC153700.028.570522
CAACGCA156400.027.8921245
AACGCAG164300.026.804266
TCTGTCG11750.025.5980288
TAACGCA6200.025.021024
GTACATG86200.024.599181
ACCTGGG49900.024.5890353
CTGTCGC13700.024.3557879
ACGCAGA180900.024.1090477
CGCAGAG181350.023.9970478
Produced by FastQC (version 0.11.5)