FastQCFastQC Report
Thu 2 Jun 2016
DRR025039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3484934
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108850.3123445092503904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90920.26089446744185113No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63280.1815816311011916No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79950.041.4171561
GTATCAA134350.035.8499341
TACCTGG36800.032.1906282
ATAACGC4550.032.027793
GTACCTG37800.031.9791871
TATAACG4550.030.9946372
ATCAACG162950.029.1369233
TCAACGC163900.028.9036274
ACAACGC5750.028.613893
CAACGCA168500.028.1424565
TATCAAC170200.028.0614992
AACGCAG176900.027.0452336
TAACGCA5300.026.6021124
GTACATG84750.025.3076151
TCTGTCG13400.025.251438
ACGCAGA195350.024.4183947
CGCAGAG195900.024.1095948
TACATGG84100.023.8116932
ACCTGGG50050.023.1990153
CTGTCGC15350.022.9620789
Produced by FastQC (version 0.11.5)