FastQCFastQC Report
Thu 2 Jun 2016
DRR025038_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025038_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262396
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150690.28635245238100665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125640.23875056153128726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76510.14539004666315494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99100.054.5103951
GTATCAA183250.041.331821
ATCAACG226300.032.926433
TCAACGC229800.032.4847684
TATCAAC233150.032.061082
CAACGCA243950.030.5611215
AACGCAG253400.029.6400786
GTGGTAT44650.027.4822311
ACGCAGA285100.025.9477777
CGCAGAG286600.025.5987858
GTACATG206100.025.4120791
TGGTATC47850.024.4643612
TACATGG209600.023.9998742
GCAGAGT302300.023.5230399
ACATGGG216050.022.390363
TATAACG5300.021.2888322
GAGTACT183150.020.82359312-13
CAGAGTA294950.020.24505210-11
CATGGGG124800.019.9260124
AGAGTAC280800.019.71700110-11
Produced by FastQC (version 0.11.5)