FastQCFastQC Report
Thu 2 Jun 2016
DRR025038_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025038_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5262396
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171510.3259161796261627No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145860.2771741237261506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101240.1923838494860516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104700.055.1240271
GTATCAA190500.041.827461
ATCAACG245700.032.1257483
TCAACGC251200.031.5084484
TATCAAC254600.031.1141172
CAACGCA260200.030.4366685
GTGGTAT46350.029.1256221
AACGCAG272650.029.0982826
TGGTATC47050.027.778012
ACGCAGA311100.025.2441257
CGCAGAG312200.025.0636658
GTACATG205350.023.7305681
GCAGAGT332650.023.0992369
TACATGG210050.022.4712372
TATAACG5300.021.2888092
ACATGGG214150.021.2283443
TACCTGG43350.020.9307212
GAGTACT213750.020.52464112-13
AGAGTAC311050.020.42743710-11
CAGAGTA324650.019.82503710-11
Produced by FastQC (version 0.11.5)