FastQCFastQC Report
Thu 2 Jun 2016
DRR025037_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025037_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469197
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164630.36836595030382413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137500.3076615329330974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84000.18795322739185585No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96750.056.520991
GTATCAA180900.043.354931
ATCAACG226700.034.3443
TCAACGC229250.033.9194534
TATCAAC235450.033.1701242
CAACGCA242500.032.084795
AACGCAG253850.030.7366396
GTGGTAT39600.029.8029691
GTACATG213400.027.5420741
CGCAGAG279700.027.4904778
ACGCAGA281800.027.3693147
TGGTATC41600.027.3525712
TACATGG209600.026.8521462
GCAGAGT298050.025.1827059
ACATGGG216050.025.0909523
TACCTGG38700.025.028362
GTACCTG44150.022.3650361
CATGGGA108800.021.6054734
GAGTACT183350.021.3778712-13
CAGAGTA292800.021.15587210-11
Produced by FastQC (version 0.11.5)