FastQCFastQC Report
Thu 2 Jun 2016
DRR025037_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025037_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4469197
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177650.3974987005495618No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153210.3428132615322171No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103750.23214461121315527No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96950.056.1839751
GTATCAA179200.041.9197461
ATCAACG232700.032.0189823
GTGGTAT42250.031.841321
TCAACGC237300.031.4117834
TGGTATC43150.030.8308222
TATCAAC242250.030.7958832
CAACGCA247300.030.1035865
AACGCAG259550.028.7733236
GTACATG211600.025.697681
ACGCAGA299400.024.754687
CGCAGAG297150.024.6726938
TACATGG212600.024.5878772
TACCTGG37550.023.1601432
ACATGGG217400.023.0716933
GCAGAGT312200.022.8661259
GTACCTG43350.020.182531
CATGGGA106450.020.0004444
AGAGTAC299550.019.9565510-11
GAGTACT197150.019.72599612-13
Produced by FastQC (version 0.11.5)