FastQCFastQC Report
Thu 2 Jun 2016
DRR025036_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025036_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418502
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119740.35027038158819274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100130.29290607406401986No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61050.17858699512242496No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66600.053.371031
GTATCAA123100.041.975671
TACCTGG33500.033.40142
ATCAACG154150.033.0878033
TCAACGC154350.033.0439644
TATCAAC159100.032.2394142
CAACGCA162750.031.2509045
AACGCAG169350.029.9457656
GTACCTG41350.028.5401461
GTACATG134600.028.468891
GTGGTAT28450.027.2684021
TACATGG134950.027.2088832
ACGCAGA192300.026.1511577
ACCTGGG42350.025.8633173
CGCAGAG193900.025.6679928
ACATGGG139400.025.055773
TGGTATC30800.023.812582
GCAGAGT206500.023.2824849
CATGGGA65800.022.2179264
AGAGTAC192400.020.38426210-11
Produced by FastQC (version 0.11.5)