FastQCFastQC Report
Thu 2 Jun 2016
DRR025036_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025036_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418502
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135320.3958458997537518No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116020.3393884221802415No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79350.232119214790572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68900.054.8052641
GTATCAA128900.042.3915371
ATCAACG168100.032.297013
TCAACGC169900.032.0050164
TATCAAC172150.031.5644912
TACCTGG33950.031.1523442
CAACGCA177700.030.6530825
GTGGTAT27400.029.5191481
AACGCAG185850.029.4348746
GTACCTG38500.028.5812421
ACCTGGG39200.026.2606873
TACAACG7350.026.2207152
ACGCAGA212900.025.517317
GTACATG131500.025.3539961
TGGTATC31450.025.2588792
CGCAGAG213500.025.1814588
TACATGG131200.024.7924962
ACAACGC7350.023.6625983
GCAGAGT229100.022.9334539
ACATGGG135000.022.8411563
Produced by FastQC (version 0.11.5)