FastQCFastQC Report
Thu 2 Jun 2016
DRR025035_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025035_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4434258
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111630.25174448577417013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98690.22256260235647093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56210.1267630345370071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77150.049.423361
GTATCAA135250.038.8991551
ATCAACG165700.031.5507743
TCAACGC167000.031.3889184
CAACGCA174750.029.9158025
TATCAAC176600.029.6576652
AACGCAG182700.028.711476
GTGGTAT39850.025.9562361
GTACATG131950.025.476751
ACGCAGA205300.025.2512057
TGGTATC39700.025.2250232
CGCAGAG205700.024.996468
TACATGG132200.023.8634682
TATAACG4200.023.5078262
ATAACGC5150.022.822363
ACATGGG133850.022.549893
GCAGAGT223400.022.3217229
GTACCTG38750.020.1411251
TACCTGG34300.020.0124932
GAGTACT146850.019.89060612-13
Produced by FastQC (version 0.11.5)