FastQCFastQC Report
Thu 2 Jun 2016
DRR025035_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025035_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4434258
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120550.27186059088127035No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105650.2382585767449706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66820.15069037480453323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.052.408711
GTATCAA141100.039.3975371
ATCAACG174700.031.3735583
TCAACGC176600.031.0580834
CAACGCA183800.029.8925845
TATCAAC185150.029.70472
AACGCAG193350.028.5856346
ACGCAGA218100.025.2121567
GTGGTAT37350.025.183611
CGCAGAG217800.025.0736878
GTACATG126400.024.3337631
TACATGG126550.023.4013792
TGGTATC38650.022.7433872
GCAGAGT235900.022.492419
ACATGGG131750.021.2287523
AGAGTAC216550.020.3570410-11
GAGTACT161650.020.23501412-13
CAGAGTA230400.019.80645410-11
ATAACGC4950.018.9925373
AGTACTT168600.018.85732812-13
Produced by FastQC (version 0.11.5)