FastQCFastQC Report
Thu 2 Jun 2016
DRR025034_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025034_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6800656
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122790.1805561110575215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97550.14344204441453884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94450.053.2655561
GTATCAA179800.041.343461
ATCAACG213450.034.271523
TCAACGC215350.033.9242364
TATCAAC227000.032.2074552
CAACGCA228400.031.8614885
AACGCAG238500.030.5858866
ACGCAGA261800.027.4131167
CGCAGAG263950.027.0107698
GTACATG195600.025.287831
TACATGG190400.024.1997592
GCAGAGT288150.023.9429289
GTGGTAT47450.023.3851661
ACATGGG197250.022.5711083
TGGTATC47600.021.631622
GAGTACT181100.020.74754312-13
AGAGTAC262100.020.65658410-11
CAGAGTA280600.020.24937610-11
AGTACTT191550.019.00228912-13
GTACTTT203450.018.1798414-15
Produced by FastQC (version 0.11.5)