FastQCFastQC Report
Thu 2 Jun 2016
DRR025034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6800656
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134020.19706922391016396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112780.1658369427890486No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75840.11151865349460406No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95950.050.1449551
GTATCAA185700.042.4481431
ATCAACG232050.033.4850923
TCAACGC234500.033.0700044
TATCAAC246550.031.6685412
CAACGCA246400.031.4537985
AACGCAG257800.030.0993636
ACGCAGA293050.026.2691577
CGCAGAG296650.025.7594998
GTACATG200650.024.846391
GTGGTAT50950.024.5546671
TACATGG198250.023.9007052
TGGTATC48700.023.8413812
GCAGAGT320200.023.3807729
ACATGGG204400.021.8935533
AGAGTAC298500.020.69572610-11
GAGTACT203050.019.78916712-13
AGTACTT217200.019.50609412-13
CAGAGTA316000.018.73906110-11
CCGGTCG5800.017.8262129
Produced by FastQC (version 0.11.5)