FastQCFastQC Report
Thu 2 Jun 2016
DRR025033_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025033_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2990599
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94510.3160236460989922No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71760.2399519293626461No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51150.1710359697171035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61500.052.9775921
GTATCAA119350.043.8436621
TACCTGG29650.041.3842772
GTACCTG32750.038.7602461
ATCAACG146700.035.3444633
TCAACGC149050.035.0377544
TATCAAC154200.033.903142
CAACGCA157000.033.382195
ACCTGGG36000.033.1670763
AACGCAG165450.031.7877226
ACGCGTT300.00415904531.33844848-49
GTACCGG3500.030.8954051
TATAACG3950.028.5649662
ACGCAGA183150.028.3036797
CGCAGAG182700.028.1161548
ACAACGC7900.027.3720113
GCAGAGT189000.026.3081879
GTACATG123300.026.0430411
GTGGTAT27900.025.782281
TACATGG118950.025.0578842
Produced by FastQC (version 0.11.5)