FastQCFastQC Report
Thu 2 Jun 2016
DRR025033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2990599
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99340.33217425672917034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81290.2718184550987946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59850.2001271317217721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62500.054.6285131
GTATCAA118050.041.710311
TACCTGG31300.039.9488142
GTACCTG33600.037.5109671
ACAACGC6300.035.8151933
GTACCCG2550.033.1967161
ACCTGGG36900.032.8669933
TATAACG4000.031.7300242
GTACACG1807.2759576E-1231.3524531
ATCAACG156950.031.0587583
TCAACGC158450.030.789254
TATCAAC164050.029.6285882
CAACGCA166150.029.50385
GTACCGG3800.028.4647251
AACGCAG175100.028.156336
TAACGCA4950.025.6361354
ATAACGC5400.025.2447383
GTACATG127400.024.769421
TGGTATC28950.024.6809372
ACGCAGA197600.024.639427
Produced by FastQC (version 0.11.5)