FastQCFastQC Report
Thu 2 Jun 2016
DRR025032_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025032_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4287714
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86270.2012027854469771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73530.17148998277403765No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42910.10007663757424119No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61050.053.137531
GTATCAA114800.039.9710851
ATCAACG135300.033.4603923
TCAACGC135850.033.3575824
CAACGCA144100.031.4151765
TATCAAC145300.031.2237072
AACGCAG151500.029.9399686
ACGCAGA173250.025.7722057
CGCAGAG172450.025.6728298
GTACATG118800.023.1909831
GCAGAGT183750.023.1727059
GTGGTAT31950.021.9256971
TACATGG119350.021.8621732
TATAACG4000.021.1560842
ACATGGG118950.020.749013
AGAGTAC170750.020.1891410-11
GAGTACT121250.020.0005212-13
CAGAGTA182350.019.78112610-11
TAGCGCG1456.595809E-419.4518284
GAGTACA94800.019.3912771
Produced by FastQC (version 0.11.5)