FastQCFastQC Report
Thu 2 Jun 2016
DRR025032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4287714
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90850.21188446804054564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.1842007186113626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50010.11663557783938014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64050.055.95281
GTATCAA118950.039.7758561
ATCAACG145500.032.1468433
TCAACGC146700.031.940154
CAACGCA152600.030.7668385
TATCAAC156000.029.9831142
AACGCAG159300.029.5318166
ACGCAGA182200.025.6134287
CGCAGAG182400.025.2498028
GTACATG119650.024.9208341
TACATGG121600.023.311062
GCAGAGT197800.022.737489
ACATGGG119800.022.5233673
GTGGTAT32050.020.8375341
AGAGTAC181400.020.2205210-11
GAGTACT132200.020.17395612-13
CAGAGTA193100.020.02969210-11
TGGTATC30100.019.678062
AGTACTT136750.019.12469512-13
GTCGTAC2502.414672E-718.8124771
Produced by FastQC (version 0.11.5)