FastQCFastQC Report
Thu 2 Jun 2016
DRR025031_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025031_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4478734
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92050.2055268296799944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77440.17290600424137714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45280.10109999834774738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70950.055.266781
GTATCAA133300.040.8440671
ATCAACG160350.033.3634153
TCAACGC162500.033.006574
TATCAAC170000.031.666662
CAACGCA171850.031.156045
AACGCAG180650.029.8425086
ACGCAGA201400.026.3224077
GTACATG149800.026.3016361
CGCAGAG201800.026.0833348
TACATGG149250.025.13822
GCAGAGT216450.023.4928639
ACATGGG155600.023.08243
GTGGTAT34100.023.0256881
TGGTATC32900.022.4362052
GAGTACT132700.020.94847912-13
CAGAGTA212100.020.14150210-11
TATAACG3750.020.060162
AGAGTAC198750.019.96922310-11
AGTACTT140200.018.55403112-13
Produced by FastQC (version 0.11.5)