FastQCFastQC Report
Thu 2 Jun 2016
DRR025031_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025031_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4478734
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103070.2301319971224011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89310.19940902942661923No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58540.13070657913597905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78650.052.0942761
GTATCAA139950.039.864141
ATCAACG175050.031.367833
TCAACGC176550.031.1455364
TATCAAC184500.029.9398842
CAACGCA184900.029.8152755
AACGCAG195950.028.229246
GTACATG152200.024.94191
ACGCAGA224400.024.4402897
CGCAGAG223300.024.2868148
TACATGG153250.023.7130622
GTGGTAT37750.023.1775021
ACATGGG154750.022.4804423
TGGTATC38100.021.9636062
GCAGAGT242550.021.8167769
AGAGTAC220250.020.39836710-11
TATAACG4150.020.3907412
TACCTGG33350.019.7351682
GAGTACT151850.019.40466712-13
AGTACTT161500.019.0454212-13
Produced by FastQC (version 0.11.5)