FastQCFastQC Report
Thu 2 Jun 2016
DRR025030_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025030_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152741
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT142240.4511629721566091No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116700.37015409765661056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77110.24458082665211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77650.053.400591
GTATCAA143000.043.232331
ATCAACG185400.032.9623453
TATCAAC187400.032.687892
TCAACGC187600.032.5491874
GTGGTAT33850.030.9717041
CAACGCA198900.030.6758675
AACGCAG205200.029.8691396
TGGTATC35550.028.564472
ACGCAGA224350.026.8992867
TATAACG3850.026.8642042
CGCAGAG230600.026.087468
GTACATG162350.025.0196021
GCAGAGT237650.024.4038589
TACATGG165250.024.15342
TACCTGG29250.023.6267242
TAACGCA3900.022.9009154
ACATGGG170600.022.2371433
GAGTACT143650.021.29886812-13
AGAGTAC223150.020.88240210-11
Produced by FastQC (version 0.11.5)