FastQCFastQC Report
Thu 2 Jun 2016
DRR025030_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025030_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3152741
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158560.5029274526515182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132730.42099874363292134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94810.30072245071827974No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36750.11656523640857273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA83200.053.0816881
GTATCAA149950.041.904671
ATCAACG199300.031.2499393
TCAACGC201150.030.8875264
TATCAAC203850.030.690782
GTGGTAT34450.029.8990461
CAACGCA211400.029.4788285
AACGCAG219650.028.4144046
TGGTATC37850.025.9552
TATAACG3100.025.776732
ACGCAGA243800.025.4648327
CGCAGAG246800.024.9839088
GTACATG171450.023.8662511
GCAGAGT258600.023.2623279
TAACGCA4050.023.2084794
TACATGG172350.023.1000392
TACAACG7350.023.0226732
TACCTGG26850.022.5832272
ACATGGG175250.021.725393
AGAGTAC244650.021.26542910-11
Produced by FastQC (version 0.11.5)