FastQCFastQC Report
Thu 2 Jun 2016
DRR025029_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameDRR025029_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4780995
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118560.24798185314981505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101010.2112740130454016No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59500.12445108183547567No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99700.046.405061
GTATCAA168600.037.7037471
ATCAACG206250.030.3379253
TCAACGC208150.030.1045954
TATCAAC216050.029.0736372
CAACGCA219750.028.4076045
AACGCAG231550.027.1200666
GTACATG187200.026.2971
TACATGG187900.024.8217332
ACGCAGA254150.024.4477357
CGCAGAG256300.024.0215938
GTGGTAT50750.023.4395831
ACATGGG191100.023.4195213
GCAGAGT273100.021.9585449
TGGTATC54700.020.6286642
GAGTACT156400.020.147912-13
AGAGTAC243600.019.75602110-11
TACCTGG36400.019.3747942
CATGGGG107150.019.2597854
CAGAGTA266700.019.09337810-11
Produced by FastQC (version 0.11.5)